Journal: bioRxiv
Article Title: Curcumin Alleviates Systemic Inflammation and Gut Dysbiosis Induced by Circadian Rhythm Disruption in a Rodent Model of Jet Lag
doi: 10.64898/2026.01.18.698756
Figure Lengend Snippet: Schematic in the left panel shows that animals were individually housed in cages equipped with running wheels, and wheel revolutions were monitored daily using automated software. Rats were fed either curcumin or almond butter (vehicle) diet balls. Schematic in the right panel shows the timeline and overview of experimental procedures. Following behavioral testing, blood samples were collected for ELISA-based cytokine and chemokine analysis. Small intestine segments were harvested for Peyer’s patch analysis using ImageJ. Fecal samples were collected for microbiome analysis.
Article Snippet: Quantikine® high-sensitivity colorimetric sandwich ELISA kits (R&D Systems) were used to quantify levels of tumor necrosis factor alpha (TNF-α; RTA00), interferon gamma (IFN-γ; RIF00), interleukin-6 (IL-6; R6000B), cytokine-induced neutrophil chemoattractant-1 (CXCL1/CINC-1; RCN100), and interleukin-4 (IL-4; R4000).
Techniques: Software, Enzyme-linked Immunosorbent Assay